Biocompatibility

For the application of novel biomaterials and coatings for biomedical implants, it is important to ensure the biocompatibility of these materials. In our lab, we utilize in vitro cell viability studies on application-specific cell lines to determine the biocompatibility of our materials, and the materials of our collaborators, at early stages. Most frequently, we perform MTT assays, which are a colorimetric assay for assessing cell metabolic activity. In addition, we qualitatively assess the cell viability by cell staing.

Below are the results from our biocompatibility study of Pt-based metallic glasses, which is a collaborative project with Dr. Kumar and his Advanced Materials and Manufacturing Lab from UT Dallas.

Fig. 1. (a) Viability of cells cultured on MGs after 24 h and 48 h. (b) The fluorescent images of L-929 cells cultured for 48 h on MGs and stained with ProLong™ Gold Antifade Mountant with DAPI (nuclei, blue) and ActinGreen™ 488 ReadyProbes™ Reagent (F-actin, green). [1]

[1] S. Jagdale, Q. Hu, M. Ecker, G. Kumar; Biocompatibility and thermoplastic formability of Pt-based metallic glasses, Materials Letters, 2021, 295, 129870. https://doi.org/10.1016/j.matlet.2021.129870